Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p099 | (1) | WCRB2014

Selection of reference genes in mouse preimplantation embryos of different ploidies at various developmental stages

Gu Yanli , Shen Xinghui , Zhou Dongjie , Wang Zhendong , Lei Lei

Real-time RT quantitative PCR (qPCR) has become the most frequently used system for studies of gene expression. Many studies have provided reliable evidences that the transcription levels of reference genes are not constant at different developmental stages and in different experimental conditions. However, suitable reference genes which are stably expressed in polyploid preimplantation embryos of different developmental stages have not yet been identified. In the present stud...